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Enspire Plate Reader Software Say Overflow
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Dont try to use conversion factors, equivalences, or other mathematical manipulation of data.

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Join for free Log in Most recent answer 25th Feb, 2021 Hawnaz Najmalddin University of Sulaimani Dear Irene, I know this question has been posted along time ago, but I would like to add an answer to this question as other researcher may find it useful.

I had the same problem, I figured out that it was because of the occurrence of high concentration of the molecule I am investigating.

Cite All Answers (23) 23rd May, 2013 Aleksander Balter Which model of a spectrofluorometer are you using Cite 23rd May, 2013 Irene Cannavaro Politecnico di Torino LS 55 Fluorescence Spectrometer.

Im using quinine sulphate samples with a concentration of few ppb.

Enspire Plate Reader Software Say Overflow

I tried to change the method and also to shut off everything but after the first measure the software say overflow Cite 23rd May, 2013 Irene Cannavaro Politecnico di Torino from Perkin Elmer, I forgot Cite 23rd May, 2013 Aleksander Balter Sorry, but I have no idea what may cause such an error message (we are using an older Perkin Elmer LS 50B model) Cite 23rd May, 2013 Irene Cannavaro Politecnico di Torino Thank you for trying Cite 23rd May, 2013 Nikodem Czechowski Most probably this error is caused by an excessive illumination of the detector.

In which conditions you see such an error Cite 23rd May, 2013 Oleksandr Savsunenko Titanovo, Inc No, vice versum.

Excessive illumination in not possible in UVVis spectroscopy.

Try dilution, recheck wavelength an make additional precautions if working in 180nm-210nm area Cite 24th May, 2013 Harry ten Brink ECN part of TNO Indeed, you should add information at what situations you get this message Cite 3rd Jun, 2013 Irene Cannavaro Politecnico di Torino Dear all, i dont think that the error is due to excessive illumination because the instrument works for the first measure without any problems and after that it dont work and appear the error overflow.

Someone said me that i need to rename the method with a short name, but i think that this is a not scientific solution I worked almost in all condition (opening the slits or closing them in order to avoid signal saturation) but with no results.

I dont think that is a problem of my samples but i think its an instrument problem.

The slits were both set at 4.0 Cite 3rd Jun, 2013 Aleksander Balter Irene, you can try to contact Perkin Elmer technical support: I hope they will be able to help resolve your problem.

Can you set the number of points, resolution or whatever increases the size of the data or the aquisition rate This may be related to an overflow in the aquisition cards or analog to digital conversion hardware.

After the first aquisition of a data set to big to processed a second try results in the overflow.

Every time your sample has a fluorescence above 1000, you will get an overflow message.

The solution is simple: you can (a) use a lower slit value (2.5 nm, for instance), (b) use a lower voltage of the photomultiplier, (c) both.

If your sample is very, very fluorescence you can always dilute it.

Keep in mind that you have to select one with a wavelenght between your excitation and emission wavelengths.

The last option is to select the filter called 1 transmitance.

This is the very last choice because it makes all your readings very low and you will loose sensitivity.

Very important note: since fluorescence increases exponentially with the voltage of the photomultiplier, ALL your readings have to be performed using the same voltage.

If one of your samples exceeds the scale of the fluorometer, setup the equipment again as I explained before, and read all your samples again.

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